2. Apoptosis Epigenetics Cell Cycle/DNA Damage MAPK/ERK Pathway Anti-infection Autophagy
  3. Bcl-2 Family Caspase PARP p38 MAPK Parasite Autophagy
  4. Dehydrocorydaline chloride

Dehydrocorydaline chloride  (Synonyms: 盐酸脱氢紫堇碱; 13-Methylpalmatine chloride)

目录号: HY-N0674A 纯度: 99.72%
COA 产品使用指南

摩尔计算器

Dehydrocorydaline chloride (13-Methylpalmatine chloride) 是一种生物碱。Dehydrocorydaline 调节 Bax，Bcl-2 蛋白表达；激活 caspase-7，caspase-8，并使 PARP 失活。Dehydrocorydaline chloride 能增强 p38 MAPK 活化，具有抗炎、抗癌等功效。Dehydrocorydaline chloride 具有强大的抗疟疾作用，并具低细胞毒性 (细胞生存力> 90％)， P. falciparum 3D7 strain (IC50=38 nM)。

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Dehydrocorydaline chloride Chemical Structure

Dehydrocorydaline chloride Chemical Structure

CAS No. : 10605-03-5

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥1540
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1 mg ￥533
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5 mg ￥1400
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10 mg ￥2000
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Customer Review

Other Forms of Dehydrocorydaline chloride:

Dehydrocorydaline chloride 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 28 篇科研文献

WB

    Dehydrocorydaline chloride purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2018 May 23;499(4):743-750.  [Abstract]

    Astrocytes are cultured with p38 and JNK activator of DHC and ANS, respectively, at the indicated concentrations for 24 h. Then, all cells are harvested for western blot analysis of p-p38 and p-JNK. Astrocytes are pretreated with DHC (500 nM) or ANS (10 mM) for 24 h, followed by Fru (5 mM) for an additional 24 h.

    Dehydrocorydaline chloride purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2018 Sep 5;503(2):467-473.  [Abstract]

    The increase of Bcl-2 and decrease of Bax, cleaved Caspase-3 and PARP induced by Ulk1- inhibition in LPS-exposed AST are abrogated by the Dehydrocorydaline chloride (DHC) or Anisomycin (ANS) pre-treatment

    查看 Bcl-2 Family 亚型特异性产品:

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    Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

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    Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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    PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

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    p38 MAPK p38α p38β MAPK13 p38δ p38γ

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    Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Dehydrocorydaline chloride (13-Methylpalmatine chloride) is an alkaloid that regulates protein expression of Bax, Bcl-2; activates caspase-7, caspase-8, and inactivates PARP[1]. Dehydrocorydaline chloride elevates p38 MAPK activation. Anti-inflammatory and anti-cancer activities[2]. Dehydrocorydaline chloride shows strong anti-malarial effects (IC50 =38 nM), and low cytotoxicity (cell viability > 90%) using P. falciparum 3D7 strain[3].

    IC50 & Target

    Plasmodium

     

    体外研究
    (In Vitro)

    Treatment of C2C12 myoblasts with 500 nM Dehydrocorydaline increases the expression levels of muscle-specific proteins, including MyoD, myogenin and myosin heavy chain. Treatment with Dehydrocorydaline elevates p38 MAPK activation and the interaction of MyoD with an E protein. Furthermore, defects in differentiation-induced p38 MAPK activation and myoblast differentiation induced by depletion of the promyogenic receptor protein Cdo in C2C12 myoblasts are restored by Dehydrocorydaline treatment[2]. Dehydrocorydaline significantly inhibits MCF-7 cell proliferation in a dose- dependent manner, which can be reversed by a caspase-8 inhibitor, Z-IETD-FMK. Dehydrocorydaline increases DNA fragments without affecting ΔΨm. Western blotting assay shows that dehydrocorydaline dose-dependently increases Bax protein expression and decreases Bcl-2 protein expression. Furthermore, dehydrocorydaline induces activation of caspase-7,-8 and the cleavage of PARP without affecting caspase-9. These results show that dehydrocorydaline inhibits MCF-7 cell proliferation by inducing apoptosis mediated by regulating Bax/Bcl-2, activating caspases as well as cleaving PARP[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Dehydrocorydaline chloride 相关抗体:
    体内研究
    (In Vivo)

    Dehydrocorydaline (3.6, 6 or 10 mg/kg, i.p.) shows a dose-dependent antinociceptive effect in the acetic acid-induced writhing test and significantly attenuates the formalin-induced pain responses in mice. In the formalin test, dehydrocorydaline decreases the expression of caspase 6 (CASP6), TNF-α, IL-1β and IL-6 proteins in the spinal cord. These findings confirm that Dehydrocorydaline has antinociceptive effects in mice[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    401.88

    Formula

    C22H24ClNO4
    CAS 号
    性状

    固体

    颜色

    Light yellow to yellow

    中文名称

    盐酸脱氢紫堇碱；盐酸去氢紫堇碱；盐酸脱氢紫堇硷；盐酸脱氢延胡索碱；盐酸去氢延胡索碱
    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : 25 mg/mL (62.21 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.4883 mL 12.4415 mL 24.8830 mL
    5 mM 0.4977 mL 2.4883 mL 4.9766 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (6.22 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

      生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (6.22 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

      20% SBE-β-CD in Saline 的配制（4°C，储存一周）：2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    请输入您的动物体内配方组成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
    连续给药周期超过半月以上，请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.72%

    COA (266 KB) HNMR (286 KB) LCMS (255 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [1]

    Briefly, MCF-7 cells (1×104 cells/well) are seeded in 96-well plates and treated with different concentrations of dehydrocorydaline (0-200 μM) for 24 h. The cell viability is determined. To explore the role of caspase-8 in dehydrocorydaline induced cytotoxicity, a caspase-8 inhibitor Z-IETD-FMK (10 μM) is co-incubated with 200 μM dehydrocorydaline.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [4]

    Briefly, the mice are placed individually in glass beakers and are allowed to acclimate for 30 min before the test. The vehicle or Dehydrocorydaline (3.6, 6 or 10 mg/kg) are injected (10 ml/kg, i.p.) 15 min prior to the formalin injection. Morphine (10 mg/kg) or diclofenac sodium (20 mg/kg) are injected 15 and 30 min, respectively, prior to the formalin injection as positive controls. Then, 25 μL of a 5% formalin solution is injected into the plantar surface of the right hind paw of each mouse. Immediately after the formalin injection, the mice are placed individually in the beakers, and a mirror is placed under the beaker to allow clear observation of the paws of the animals. The time that the animals spent on biting/licking the injected paw is measured with a stopwatch every 5 min and considered as indication of nociception.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Dehydrocorydaline chloride 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.4883 mL 12.4415 mL 24.8830 mL 62.2076 mL
    5 mM 0.4977 mL 2.4883 mL 4.9766 mL 12.4415 mL
    10 mM 0.2488 mL 1.2442 mL 2.4883 mL 6.2208 mL
    15 mM 0.1659 mL 0.8294 mL 1.6589 mL 4.1472 mL
    20 mM 0.1244 mL 0.6221 mL 1.2442 mL 3.1104 mL
    25 mM 0.0995 mL 0.4977 mL 0.9953 mL 2.4883 mL
    30 mM 0.0829 mL 0.4147 mL 0.8294 mL 2.0736 mL
    40 mM 0.0622 mL 0.3110 mL 0.6221 mL 1.5552 mL
    50 mM 0.0498 mL 0.2488 mL 0.4977 mL 1.2442 mL
    60 mM 0.0415 mL 0.2074 mL 0.4147 mL 1.0368 mL
    Help & FAQs
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Dehydrocorydaline10605-03-513-Methylpalmatine盐酸脱氢紫堇碱盐酸去氢紫堇碱盐酸脱氢紫堇硷盐酸脱氢延胡索碱盐酸去氢延胡索碱Bcl-2 FamilyCaspasePARPp38 MAPKParasiteAutophagypoly ADP ribose polymeraseInhibitorinhibitorinhibit

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